Cumulative bleaching undermines systemic resilience of the Great Barrier Reef

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Identification of Novel Kinases of Tau Using Fluorescence Complementation Mass Spectrometry (FCMS)

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ppGpp is a bacterial cell size regulator

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KINEMATIC VARIABLES AND BLOOD ACID-BASE STATUS IN THE ANALYSIS OF COLLEGIATE SWIMMERS’ ANAEROBIC CAPACITY

Short duration repeated maximal efforts are often used in swimming training to improve lactate tolerance, which gives swimmers the ability to maintain a high work rate for a longer period of time. The aim of the study was to examine the kinematics of swimming and its relation to the changes in blood acid-base status and potassium level. Seven collegiate swimmers, with at least 6 years of training experience, volunteered to participate in the study. The test consisted of 8 x 25 m front crawl performed with maximum effort. The rest period between repetitions was set to five seconds. Blood samples were taken from the fingertip at rest, after warm-up and in the 3rd minute after completion of the test. The swimming was recorded with a video recorder, for later analysis of time, velocity and technique (stroke index). Based on the swimming velocity results, the obtained curve can be divided into rapid decrease of velocity and relatively stable velocities. The breaking point of repetition in swimming velocity was assumed as the swimming velocity threshold and it was highly correlated with the decrease of the blood acid-base status (pH r=0.82, BE r=0.87, HCO₃ ^- r=0.76; p<0.05 in all cases). There was no correlation between stroke index or fatigue index and blood acid-base status. Analysis of the swimming speed in the 8 x 25 m test seems to be helpful in evaluation of lactate tolerance (anaerobic capacity) in collegiate swimmers.     

Deciphering Spatial Protein–Protein Interactions in Brain Using Proximity Labeling

Cellular biomolecular complexes including protein–protein, protein–RNA, and protein–DNA interactions regulate and execute most biological functions. In particular in brain, protein–protein interactions (PPIs) mediate or regulate virtually all nerve cell functions, such as neurotransmission, cell–cell communication, neurogenesis, synaptogenesis, and synaptic plasticity. Perturbations of PPIs in specific subsets of neurons and glia are thought to underly a majority of neurobiological disorders. Therefore, understanding biological functions at a cellular level requires a reasonably complete catalog of all physical interactions between proteins. An enzyme-catalyzed method to biotinylate proximal interacting proteins within 10 to 300 nm of each other is being increasingly used to characterize the spatiotemporal features of complex PPIs in brain. Thus, proximity labeling has emerged recently as a powerful tool to identify proteomes in distinct cell types in brain as well as proteomes and PPIs in structures difficult to isolate, such as the synaptic cleft, axonal projections, or astrocyte–neuron junctions. In this review, we summarize recent advances in proximity labeling methods and their application to neurobiology.     

Esophageal squamous cell carcinoma and adenocarcinoma in Malaysia – Pooled data from upper gastrointestinal centers in a multiethnic Asian population

BackgroundEsophageal cancer is the sixth leading cause of cancer death worldwide with considerable geographical histological variation There is a paucity of data in esophageal cancer in demographics, histology, and survival among the multi-ethnic Malaysian population. This paper is a review of esophageal cancer epidemiology and survival among esophageal cancer patients from data collected by the Malaysian Upper Gastrointestinal Surgical Society.MethodsThis is a multicenter retrospective observational study of esophageal cancer patients from six upper gastrointestinal surgical centers in Malaysia between 2005 and 2019. Patient characteristics, histological type and stage were compared and survival analyzed.ResultsThere were 820 patients with esophageal cancer included, where 442 (53.9 %) cases had squamous cell carcinoma (SCC) and 378 (46.1 %) had adenocarcinomas (AC). Malays were the predominant ethnicity with AC (66.7 %) while Indians were the ethnic majority (74.6 %) with SCC. Majority of patients (56.8 %) presented as stage IV disease. Overall, the 1-, 3-, and 5-years’ survival were 35.8 %, 13.8 % and 11.0 %, respectively. Surgical resection with curative intent yielded the best 5-year survival (29.4 %). Intervention in stage IV AC yielded superior survival when compared to SCC (median survival, 7.9 months vs 4.8 months; p, 0.018) Our series demonstrated an increase in AC to SCC over the last 15 years.ConclusionsThere was an ethnic preponderance seen between different histology in this region, not previously discussed. An increase in AC was observed over the last 15 years. Late diagnosis seen in most patients imparts poor prognosis as curative surgery affords the best outcome.     

Free Radical Induced Degradation of 1, 2-Dibromoethane. Generation of Free Br+ Atoms

Intramolecular electron transfer in hen egg-white lysozyme between tryptophan and tyrosine units was investigated by means of pulse radiolysis in the temperature range 288–333 K. An Arrhenius plot for the kinetics of this process shows a sharp break at ～303 K (30°C) compatible with the trend noted earlier (cf P. Jolles, et al. BBA. 491. 354. (1977)) on the Arrhenius plot for kinetics of bacterial substrate digestion by lysozyme. The departure from linearity of the Arrhenius plot for intramolecular electron transfer is interpreted in terms of local intralobe fluctuations of the native structure of lysozyme. It is suggested that such an approach can be useful for probing predenaturational changes in proteins.     

The Reaction of Sulfite Radical Anion with Nucleic Acid Components

The sulfite radical anion (SO₃^?) is the first intermediate in the autoxidation of sulfite to sulfate. Using competition kinetics, its reactivities with the nucleic acid bases and the corresponding nucleosides were investigated. The second order rate constants were found to be rather low, k < 1 × 10⁶dm³mol^?1s^?1 at pH 7. As a competitor, the carotenoid crocin was used, which was found to be bleached very efficiently by SO₃^? (k = 1.0 × 10⁹dm³mol^?1S^?1).     

我国三大城市群地区城市和农业用地地表热环境效应对比研究

以大中小城市协同发展为特征的城市群已成为我国城市化发展的主要形式，城市化和农业作为城市群地区最主要的土地利用活动，其气候效应是国际研究的热点。然而过去研究多关注大城市的热岛效应，对更为普遍的农业活动以及中小城市城市化的气候效应认识十分薄弱。基于MODIS地表温度数据，以自然林地为参照，提出了一种可逐像元估算土地利用地表热环境效应的新方法，进而对比分析了我国三大城市群地区（京津冀、长三角和珠三角）城市和农业用地地表热环境效应及其驱动因子差异。结果表明各城市群白天城市热岛效应明显，地级以上城市年平均热岛强度达3.2℃以上，但最强热岛均未发生在核心城市。夜晚热岛效应明显减弱，京津冀和长三角部分城市甚至出现冷岛效应。农业用地在白天亦表现出明显的增温效应，特别是在京津冀地区，而在夜晚除珠三角城市群外，降温效应明显，京津冀和长三角地区平均降温2.3℃和0.7℃。虽然城市用地平均增温强度大于农业用地，但农业用地因面积优势对区域温度变化起控制作用。白天城市和农业用地整体导致各城市群温度明显增加，京津冀增温最高（4.0℃），夜晚二者导致长三角和京津冀地区平均温度降低。研究还发现各城市群城市和农业用地地表热环境效应时空异质性极大，主要受植被、地表反照率、气候背景和人口密度控制。本文对制定缓解气候变化的土地利用策略具有重要的指导意义。